Journal: Immunity
Article Title: microRNA-142 is critical for the homeostasis and function of type-1 innate lymphoid cells
doi: 10.1016/j.immuni.2019.06.016
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: 16% Paraformaldehyde Aq Sol., Case of 10 Fisher 50-980-489 DMEM Media Gibco 11965 DMSO Sigma D2650 EDTA Corning 46-034-CL Heparin Fresenius Kabi USA 401807K AO/PI Nexcelom CS2-0106-5ML Ethyl Alcohol 200 Proof Pharmco Cat #11100020S Critical Commercial Assays EasySepTM Mouse NK Cell Isolation Kit Stem Cell Technologies Catalog # 19855 Direct-zol RNA Microprep Zymo Research R2061 PE Annexin V Apoptosis Detection Kit BD Bioscience Catalog No. 559763 Automacs NK Cell Isolation Kit Miltenyi CellTraceTM Yellow Cell Proliferation Kit, for flow cytometry Thermo Fisher CellTraceTM Violet Cell Proliferation Kit, for flow cytometry Thermo Fisher C34557 Promega Dual-Glo Luciferase Assay System, 10mL Thermo Fisher PR-E2920 Deposited Data MicroArray GEO GSE85476 Experimental Models: Cell Lines Mouse: 3T3 ATCC Human: 293T Sands Lab Experimental Models: Organisms/Strains Mouse: Mir142 −/− Reddy Lab n/a Mouse: Mir142 f/f Bolden Lab n/a Mouse: CD45.2 C57BL/6J The Jackson Laboratory Cat# JAX: 000664 Mouse: CD45.1 C57BL/6J The Jackson Laboratory Cat# JAX: 002014 Mouse: Ncr1-icre Vivier Lab Mouse: Il15 −/− Li Lab Oligonucleotides has-miR-142-3p, S(50RT/150 PCR rxns) Life Technologies 000464 has-miR-142-5p, S(50RT/150 PCR rxns) Life Technologies 002248 snoRNA135, TaqMan microRNA control Assay, S(50RT/150 PCR rxns) Life Technologies 001230 Software and Algorithms FlowJo Treestar Inc Automacs Miltenyi Biotec Cellometer Nexcelom GraphPad Prism 8 GraphPad Image Lab BioRad Open in a separate window KEY RESOURCES TABLE miR-142 is induced by IL-15 and required for type-1 ILC development and homeostasis miR-142 targets SOCS1 thereby promotes IL-15 receptor signaling in type-1 ILCs αV integrin, a miR142-3p target, promotes IL-15-independent survival in type-1 ILCs miR-142 is critical for cytokine production and NK cell-mediated viral control
Techniques: Virus, Recombinant, Derivative Assay, Saline, Electron Microscopy, Cell Isolation, Flow Cytometry, Luciferase, Microarray, Control, Software
